MYELOID NEOPLASIA An insertional mutagenesis screen identifies genes that cooperate with Mll-AF9 in a murine leukemogenesis model
نویسندگان
چکیده
1Department of Genetics, Cell Biology and Development and Department of Pediatrics, Masonic Cancer Center, University of Minnesota Twin Cities, Minneapolis, MN; 2Division of Pharmaceutical Sciences, School of Pharmacy, University of Wisconsin-Madison, Madison, WI; 3Biostatistics and Bioinformatics, Masonic Cancer Center, University of Minnesota Twin Cities, Minneapolis, MN; 4Department of Hematology, Erasmus University Medical Center, Rotterdam, The Netherlands; 5Cold Spring Harbor Laboratory, Cold Spring Harbor, NY; 6Watson School of Biological Sciences, Cold Spring Harbor, NY; 7Laboratory of Cellular Oncology, Center for Cancer Research, National Cancer Institute, Bethesda, MD; 8Howard Hughes Medical Institute, Cold Spring Harbor, NY; 9Comparative Pathology Shared Resource, Masonic Cancer Center, University of Minnesota Twin Cities, Minneapolis, MN; 10Department of Laboratory Medicine, Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, CA; and 11Experimental Cancer Genetics, The Wellcome Trust Sanger Institute, Hinxton, United Kingdom
منابع مشابه
An insertional mutagenesis screen identifies genes that cooperate with Mll-AF9 in a murine leukemogenesis model.
Patients with a t(9;11) translocation (MLL-AF9) develop acute myeloid leukemia (AML), and while in mice the expression of this fusion oncogene also results in the development of myeloid leukemia, it is with long latency. To identify mutations that cooperate with Mll-AF9, we infected neonatal wild-type (WT) or Mll-AF9 mice with a murine leukemia virus (MuLV). MuLV-infected Mll-AF9 mice succumbed...
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Identification of the targets of mixed lineage leukemia (MLL) fusion genes will assist in understanding the biology of MLL fusion gene leukemias and in development of better therapies. Numerous studies have implicated HOXA9 as one of the possible targets of MLL fusion proteins. To determine if HOXA9 was required for leukemia development by MLL fusion genes, we compared the effects of the Mll-AF...
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The cAMP response element-binding protein (CREB) is a nuclear transcription factor that is critical for normal and neoplastic hematopoiesis. Previous studies have demonstrated that CREB is a proto-oncogene whose overexpression promotes cellular proliferation in hematopoietic cells. Transgenic mice that overexpress CREB in myeloid cells develop a myeloproliferative disease with splenomegaly and ...
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Chromosomal rearrangements involving the H3K4 methyltransferase mixed-lineage leukemia (MLL) trigger aberrant gene expression in hematopoietic progenitors and give rise to an aggressive subtype of acute myeloid leukemia (AML). Insights into MLL fusion-mediated leukemogenesis have not yet translated into better therapies because MLL is difficult to target directly, and the identity of the genes ...
متن کاملCdx4 is dispensable for murine adult hematopoietic stem cells but promotes MLL-AF9-mediated leukemogenesis.
BACKGROUND Cdx4 is a homeobox gene essential for normal blood formation during embryonic development in the zebrafish, through activation of posterior Hox genes. However, its role in adult mammalian hematopoiesis has not been extensively studied and its requirement in leukemia associated with Hox gene expression alteration is unclear. DESIGN AND METHODS We inactivated Cdx4 in mice through eit...
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